Figure 1. Analysis of lymphocyte populations in DENV NS1- or NS1+ Bone marrows
Cells were collected from NS1- or NS1+ bone marrows and analyzed by multicolor flow cytometry with cell surface markers.
(A)The population of T cells including CD8+ effector T cells, Naïve T cells, effector memory T cells, central memory T cells as well as CD4+ effector T cells, Naïve T cells, effector memory T cells, central memory T cells were shown. (B) The population of B cell including Naïve B cells, Resting memory B cells, Tissue-like memory B cells, Active memory B cells, Antibody-secreting cells (ASCs). (C) Subpopulation of ASCs including plasma cells and plasmablast cells. The data was shown with mean ± SEM.
Figure 2. B cell populations after DENV2 or NS1 recombinant protein stimulation in DENV NS1 positive bone marrows
Cells were collected from DENV NS1+ bone marrows and stimulated with DENV2 (MOI=1) or NS1 recombinant protein (100 ng). Cells were collected at indicated times and subjected to FACS analysis for B cell populations. (A) Subset of ASCs, plasma cells, DENV2 or NS1 recombinant protein stimulation at indicated times. (B) Subset of ASCs, Plasmablast cells, after DENV2 or NS1 recombinant protein stimulation at indicated times. The data was shown with mean ± SEM
Figure 3. Proposed mechanism of impaired MBCs mitogenesis after DENV infection via DENV NS1 protein interact with Brd2 transcription factor