According to the Ministry of Health and Welfare in Taiwan, oral cancer is the fifth cancer deaths in Taiwan. Oral cancers are mainly associated with the usage of tobacco, betel nuts and alcohol consumption. Other risk factors include poor oral hygiene, candidiasis, viral infection, and chronic oral mucosa trauma.
With the technologies of Single-cell RNA sequencing, tumor cell invasion and metastasis between different tumor cell groups can be detected in oral cancer carcinogenesis. The clinical mucosal specimens from normal oral mucosa, oral cancer and oral precancerous patients were collected and candida infection was detected. The differences in oral mucosa carcinogenesis and between candida infection or not were analyzed.
The total cells can be distinguished into 24 cell clusters, 6 cell clusters are cancer related cells, and 18 cell cluster are immune cell cluster. The gain or loss of the cell cluster, and gene expression difference can be observed in oral mucosa carcinogenesis, and between Candida infection. The above results can be utilized to investigate the carcinogenesis of oral tumors and subcluster cells concerning to Candida infection.